Influence of transfected EGFR-cDNA on bcl-2 and Bax in glioblastoma cells.
- Author:
Zi-hui WANG
1
;
Ding YU
;
Jian-zhong HAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Glioblastoma; pathology; Humans; Nuclear Matrix; chemistry; Proto-Oncogene Proteins c-bcl-2; analysis; physiology; Receptor, Epidermal Growth Factor; genetics; physiology; Transfection; bcl-2-Associated X Protein; analysis; physiology
- From: Chinese Journal of Oncology 2003;25(3):230-233
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation of bcl-2 and Bax protein with nuclear matrix in glioblastoma cell line U87 as well as the effect of EGFR-cDNA transfection on the expression of bcl-2 and Bax in U87 cells.
METHODSThe correlation of bcl-2 and Bax protein with nuclear matrix in glioblastoma cell line U87 was studied by confocal microscopy and Western blot. The expression of bcl-2 and Bax in EGFR-cDNA transfected and untransfected glioblastoma cell lines was studied by Western blot.
RESULTSConfocal microscopic images showed that bcl-2 protein was localized in the periphery of the nuclear matrix and Bax in the nuclear matrix. A 26 kDa bcl-2 band and a specific band of Bax at about 66 000 were detected in nuclear matrix proteins by western blot. The expression of bcl-2 was lower but that of Bax was higher in EGFR-cDNA transfected cells than the control.
CONCLUSIONBcl-2 and Bax, being nuclear matrix associated proteins, are probably involved in the EGFR-cDNA induced malignant conversion of glioblastoma cells by introducing EGFR cDNA into the tumor cells.
