Establishment and characteristics of a human chordoma cell line.
- Author:
Dian-zhong ZHANG
1
;
Bao-an MA
;
Qing-yu FAN
;
Heng CHANG
;
Yan-hua WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cell Proliferation; Chordoma; genetics; pathology; Humans; Mice; Mice, Inbred BALB C; Neoplasm Invasiveness
- From: Chinese Journal of Oncology 2003;25(3):234-237
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo present an established human chordoma cell line for chordoma research.
METHODSThe specimens pathologically identified as chordoma were cultured, using primary tissue culture in vitro. The surviving cells were analyzed by morphology, histochemical stain, cell cycling analysis, karyotype analysis, electron microscopic observation, heterotransplantation and study of invasive capacity in vitro.
RESULTSThe newly established cell line CM-319 has been maintained in continual cultures for over 100 generations in two years. Its morphological observation, histochemical staining properties, electron microscopic observation and heterotransplantation showed the common characteristics of chordoma. The doubling time of cells was about 33 hours. Cell cycle analysis showed: G(1) 55.6%, G(2) 21.9% and S 22.5%, G(2)/G(1) = 1.90. Chromosome analysis showed a hypotriploid feature and the success rate of heterotransplantation was 100%. It is capable of invasion in vitro.
CONCLUSIONCM-319, as a cell line derived from human chordoma cells, may serve for further studies of chordoma.
