Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappa B pathway in human dermal fibroblasts.
10.3858/emm.2009.41.8.060
- Author:
Young Rae LEE
1
;
Eun Mi NOH
;
Eun Yong JEONG
;
Seok Kweon YUN
;
Young Ju JEONG
;
Jong Hyeon KIM
;
Kang Beom KWON
;
Byeong Soo KIM
;
Sung Ho LEE
;
Chang Sik PARK
;
Jong Suk KIM
Author Information
1. Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 560-182, Korea. jsukim@chonbuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cordycepin;
matrix metalloproteinases;
NF-kappa B;
skin aging;
ultraviolet rays
- MeSH:
Aging/physiology;
Cells, Cultured;
Deoxyadenosines/*pharmacology;
*Dermis/cytology/drug effects/physiology/radiation effects;
Dose-Response Relationship, Drug;
Enzyme Induction/drug effects;
Fibroblasts/drug effects/metabolism/radiation effects;
Gene Expression Regulation, Enzymologic;
Humans;
Infant, Newborn;
Male;
*Matrix Metalloproteinase 1/antagonists & inhibitors/biosynthesis/genetics/radiation effects;
Matrix Metalloproteinase 3/antagonists & inhibitors/*biosynthesis/genetics/radiation effects;
NF-kappa B/*antagonists & inhibitors/genetics/metabolism;
Skin/physiopathology/radiation effects;
*Ultraviolet Rays
- From:Experimental & Molecular Medicine
2009;41(8):548-554
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cordycepin (3'-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappa B activity, which was determined by I kappa B alpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappa B binding activity. However, UVB-induced NF-kappa B activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappa B activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.
