D-limonene induces apoptosis of gastric cancer cells.
- Author:
Xiao-guang LU
1
;
Bing-an FENG
;
Li-bin ZHAN
;
Zhi-hong YU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Antineoplastic Agents, Phytogenic; administration & dosage; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Cyclohexenes; Dose-Response Relationship, Drug; Humans; Proto-Oncogene Proteins c-bcl-2; metabolism; Stomach Neoplasms; metabolism; pathology; Terpenes; administration & dosage; pharmacology; Tumor Suppressor Protein p53; metabolism
- From: Chinese Journal of Oncology 2003;25(4):325-327
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis effect induced by D-limonene on BGC-823 gastric cancer cells.
METHODSThe expression of p53, bc1-2 in BGC-823 cells and qualitative, quantitative index of cell apoptosis were detected with MTT, electron microscopy, flow cytometry and immunohistochemical method.
RESULTSD-limonene could induce the formation of apoptotic bodies in a dose- and time-dependent manner. The expression of bcl-2 protein decreased and p53 protein increased in BGC-823 cells treated with D-limonene, compared with the control cells.
CONCLUSIOND-limonene exerts its cytotoxic effect on BGC-823 gastric cancer cells by inducing apoptosis.
