Expression of heparanase mRNA and its clinical significance in primary hepatocellular carcinoma.
- Author:
You-lei ZHANG
1
;
Zhi-ren FU
;
Guo-shan DING
;
Hong FU
;
Yuan-he WANG
;
Qiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Carcinoma, Hepatocellular; blood supply; enzymology; pathology; Female; Glucuronidase; biosynthesis; genetics; Humans; Liver; enzymology; Liver Neoplasms; blood supply; enzymology; pathology; Male; Microcirculation; pathology; Middle Aged; Neoplasm Invasiveness; Neovascularization, Pathologic; enzymology; RNA, Messenger; biosynthesis; genetics; Tumor Burden
- From: Chinese Journal of Oncology 2003;25(4):359-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of heparanase mRNA and its relation with the clinicopathological features and angiogenesis in primary hepatocellular carcinoma (HCC).
METHODSExpression of heparanase mRNA was detected by RT-PCR in 51 HCC lesions, and microvessel density (MVD) was detected by immunohistochemical stain with a factor VIII-related monoclonal antibody.
RESULTSExpression of heparanase mRNA was shown in 49.0% (25/51) HCC lesions. The positive rate of heparanase expression in tumors larger than 3 cm (63.6%, 21/33) was significantly higher than those in smaller tumors (22.2%, 4/18; P < 0.01). Heparanase expression was more frequent in highly invasive tumors (70.0%, 14/20) compared with moderately invasive tumors (46.7%, 7/15) and low invasive ones (25.0%, 4/16; P < 0.05). Moreover, heparanase expression in tumors with high MVD (62.5%, 20/32) was significantly higher than those in tumors with low MVD (26.3%, 5/19; P < 0.05).
CONCLUSIONHeparanase mRNA expression may be important for the growth, invasion and angiogenesis of hepatocellular carcinoma.
