Hepatitis B x protein activated vascular endothelial growth factor expression through hypoxia inducible factor-1 pathway.
- Author:
Hai-ping WANG
1
;
Xiao-ping CHEN
;
Lei DING
;
Song-qing HE
;
Muthanna ALI
;
Dong-hua LI
;
Wan-guang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Gene Expression Regulation; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Trans-Activators; physiology; Transcription Factors; genetics; physiology; Vascular Endothelial Growth Factor A; genetics; physiology
- From: Chinese Journal of Oncology 2003;25(5):433-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether hepatitis B x protein (HBx) stimulates vascular endothelial growth factor (VEGF) through hypoxia inducible factor-1 (HIF-1 alpha) pathway.
METHODSTwo plasmids including pIRES-EGFP-HBx and pTK-Hyg were co-transfected to a hepatocellular carcinoma cell line SMMC-7721. With fluorescence-positive and fluorescence-negative hygromycin-resistant colonies selected, expressions of VEGF and HIF-1 alpha in protein or/and mRNA level were detected.
RESULTSFluorescence-positive cells were stably integrated with HBx, in which expression of HIF-1 alpha and VEGF were upregulated. Fluorescence-negative cells did not express HBx, VEGF or HIF-1 alpha.
CONCLUSIONHBx can activate VEGF through HIF-1 alpha pathway.
