Rac subfamily expression and activity in gastrointestinal cancer cell lines.
- Author:
Yang-lin PAN
1
;
Feng BI
;
Na LIU
;
Jing-ping DU
;
Hai-ping JIANG
;
Yan XUE
;
Dai-ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Gastrointestinal Neoplasms; metabolism; Humans; RNA, Messenger; analysis; Reverse Transcriptase Polymerase Chain Reaction; rac GTP-Binding Proteins; genetics; rac1 GTP-Binding Protein; analysis; genetics
- From: Chinese Journal of Oncology 2003;25(5):441-444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the significance of Rac subfamily members in the gastrointestinal carcinogenesis and progression.
METHODSThe mRNA expression of Rac1, Rac2 and Rac3 in 12 kinds of gastrointestinal cancer cell lines was examined by semi-quantitative RT-PCR. The activities of Rac1 protein in 5 kinds of gastric cancer cell lines were tested by pull-down assay.
RESULTSCompared with the normal gastric mucosa and intestinal epithelial cell line, the mRNA expression of Rac1 and Rac3 was up-regulated in most of gastrointestinal cancer cell lines. The activities of Rac1 protein increased markedly in gastric cancer cell lines.
CONCLUSIONThe increased mRNA expression of Rac1 and Rac3 in gastrointestinal cancer cell lines and the abnormal activation of Rac1 protein in gastric cancer cell lines might be correlated with the carcinogenesis of gastrointestinal cancer.
