Effects of cyclosporin A on the levels of intercellular adhesion molecule-1 expressed by oral fibroblasts.
- Author:
Yunzhi FENG
1
;
Tianyou LING
;
Hanjiang WU
;
Yunliang LI
;
Zhiwen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Cyclosporine; pharmacology; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Fibroblasts; cytology; drug effects; metabolism; Humans; Immunosuppressive Agents; pharmacology; Intercellular Adhesion Molecule-1; biosynthesis; Mouth Mucosa; cytology; drug effects; metabolism
- From: Chinese Journal of Stomatology 2002;37(5):361-362
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of cyclosporin A (CSA) on the level of intercellular adhesion molecule-1 (ICAM-1) expressed by oral fibroblasts (FB).
METHODSThe fibroblasts were obtained from normal buccal mucosa (NM-FB) and cultured in vitro. Then the levels of ICAM-1 expressed by fibroblasts incubated with or without CSA in the presence of 10% fetal calf serum for 48 hours at 37 degrees C in 5% CO(2) and air were monitored by using cell-based ELISA for ICAM-1.
RESULTSWe demonstrated that NM-FB had ICAM-1 expression (A = 0.324 +/- 0.030), and after 48 hours incubated with CSA the ICAM-1 levels expressed by oral buccal mucosa fibroblasts were dose-dependently decreased.
CONCLUSIONSCSA reduces the levels of ICAM-1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.
