Polymerase chain reaction analysis of the clonality of Porphyromonas gingivalis and collagenase gene.
- Author:
Xiaozhen JIA
1
;
Mingwen FAN
;
Zhuan BIAN
;
Zhi CHEN
;
Chengzhang LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Collagenases; genetics; DNA, Bacterial; chemistry; genetics; Female; Genetic Heterogeneity; Humans; Male; Middle Aged; Periodontitis; microbiology; Polymerase Chain Reaction; methods; Porphyromonas gingivalis; genetics; isolation & purification; Sequence Analysis, DNA
- From: Chinese Journal of Stomatology 2002;37(5):363-366
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the genotypic characterization of Porphyromonas gingivalis (Pg) and the heterogeneity of a potential virulence factor-PrtC.
METHODSArbitrarily primed polymerase chain reaction (AP-PCR) was applied to 80 Pg strains isolated from 24 unrelated Chinese periodontitis patients. PCR reaction was used to detect a fragment of the collagenase gene (PrtC gene). To evaluate the sequence heterogeneity of the Pg PrtC genes, sequence analysis of four PrtC gene of clinical isolates was performed.
RESULTSRandom primer OPA-05 and OPA-17 distinguished 7 AP-PCR profiles (I through VII). The majority of the strains belonged to type VII which accounted for 25.8%. A 548bp fragment of PrtC gene was detected from 24 clinical strains. The PCR products were verified by the restriction endonucleases PstI and PvuI. Sequence analysis showed 4 PrtC genes were heterogeneous in their nucleotide composition and differed from reference strain Pg 53977.
CONCLUSIONSThe results demonstrated genetic diversity existed among these clinical strains isolated from Chinese periodontitis patients and the PrtC genes are heterogeneous in their nucleotide sequence.
