Extract and identify ingredient from Ligustrum Lucidum Ait and study its effect to periodontal pathogen.
- Author:
Qian WANG
1
;
Mingwen FAN
;
Zhuan BIAN
;
Min NIE
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Bacteroides; drug effects; Cell Division; drug effects; Ligustrum; Magnetic Resonance Spectroscopy; methods; Microbial Sensitivity Tests; Periodontium; microbiology; Plant Extracts; isolation & purification; pharmacology; Porphyromonas gingivalis; drug effects; Prevotella intermedia; drug effects; Triterpenes; pharmacology
- From: Chinese Journal of Stomatology 2002;37(5):388-390
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo extract the effective ingredient (crystal I) from effective section (saponin) of Ligustrum Lucidum Ait, identify the chemical structure of crystal I, study the effect of crystal I on P. gingivalis, B. forsythus and P. intermedia.
METHODSIsolated crystal I from saponin using the silica gel column chromatograph. Identified crystal I with IR spectra, (1)H-NMR and (13)C-NMR. Measured the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) through micro-liquid dilution. Studied the killing curve of ursolic acid on B. forsythus and P. intermedia.
RESULTSThe crystal I was identified as ursolic acid; its MIC and MBC to P. gingivalis, B. forsythus and P. intermedia were 0.740 and 0.295 microg/L respectively. The killing curve indicated that 0.800 microg/L ursolic acid could kill P. intermedia and B. forsythus in 3 and 6 hours respectively.
CONCLUSIONUrsolic acid has obvious effect to inhibit periodontal pathogen.
