Isolation, culture and identification of bovine cementum-derived cells.
- Author:
Jianxia HOU
1
;
Caifang CAO
;
Huanxin MENG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Cattle; Cell Separation; methods; Cells, Cultured; Dental Cementum; cytology; enzymology; Immunohistochemistry
- From: Chinese Journal of Stomatology 2002;37(2):102-105
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and culture bovine cementum-derived cells and investigate their biological properties.
METHODSCulture of primary bovine cementoblast (CB) were established from new-born bovine teeth. Cementum was manually dissected, fragmented, and digested twice with collagenase. Following a thorough wash to remove liberated cells, the remaining cementum fragments were plated and cultured in RPMI1640 containing 10% FCS and 1.5% Hepes. The cells in culture were identified using immunocytochemistry by expression of cementum attachment protein (CAP), osteocalcin (OCN) and alkaline phosphatase (ALP). ALP activity was measured by modified Gomori staining.
RESULTS(1) The cells in culture possessed the typical morphology of CB and there were no obvious change of the cell morphology up to 5th passage; (2) Cells in culture exhibited alkaline phosphatase activity and were also positive to CAP, OPN and ALP immunohistochemistry staining.
CONCLUSIONThe cells isolated and cultured in this experiment possess the properties of CB. This enables us to further observe its biological characteristics during cementogenesis and periodontal regeneration.
