Transforming growth factor beta(1) and bone morphogenetic protein 2 induce the differentiation of odontoblasts in vitro.
- Author:
Mingwen FAN
1
;
Qi ZHU
;
Zhuan BIAN
;
Qi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; pharmacology; Cell Differentiation; drug effects; Heparin; pharmacology; Mice; Odontoblasts; cytology; drug effects; Transforming Growth Factor beta; pharmacology; Transforming Growth Factor beta1
- From: Chinese Journal of Stomatology 2002;37(2):106-108
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of transforming growth factor beta(1) (TGF-beta(1)) and bone morphogenetic protein 2 (BMP2) combined with heparin on odontoblast differentiation.
METHODSTrypsin-isolated dental papillae from day 17 mandibular first molar were cultured in semisolid-agar medium for 6d. Recombinant human TGF-beta(1) and BMP2 combined with heparin were added to the medium.
RESULTSTGF-beta(1) and BMP2 combined with heparin induced differentiation of odontoblasts and promoted matrix secretion. Odontoblast differentiation never occurred when TGF-beta(1) or BMP2 were added alone to the medium, whereas an increase in extracellular matrix production was observed.
CONCLUSIONThese results demonstrate that both TGF-beta(1) and BMP2 stimulate the cytological and functional differentiation of preodontoblasts, and that heparin might play important role as a substrate.
