Effects of hexamethylene bisacetamide on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1.
- Author:
Lijing WANG
1
;
Jing WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetamides; pharmacology; Antineoplastic Agents; pharmacology; Apoptosis; genetics; Carcinoma, Mucoepidermoid; drug therapy; metabolism; pathology; Cell Differentiation; drug effects; Cell Division; drug effects; Gene Expression; drug effects; Genes, bcl-2; Humans; Proliferating Cell Nuclear Antigen; analysis; RNA, Messenger; analysis; Tumor Cells, Cultured
- From: Chinese Journal of Stomatology 2002;37(2):120-122
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of hexamethylene bisacetamide (HMBA) on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1.
METHODSMEC-1 was induced 72 hours by 0.002 mol/L HMBA in vitro. The morphologic observation and the expression of PCNA, bcl-2, bax protein and bcl-2 mRNA were determined by microscope, immunohistochemistry, in situ hybridization and image analysis.
RESULTSThe expression of bax, bcl-2 protein and bcl-2 mRNA were higher than control group (P < 0.01). The related genes of cell proliferating PCNA were decreased in control group.
CONCLUSIONSHMBA could inhibit the MEC-1 cell proliferation by down-regulation of PCNA expression, it could also induce apoptosis effectively that might be through the way of up-regulation of bax and bcl-2 gene expression.
