The activity of M-phase promoting factor in oral normal tissue and tumor.
- Author:
Yi LIU
1
;
Yulou TIAN
;
Aiming YU
;
Ying LIU
;
Zhihong ZONG
;
Bingzhi YU
Author Information
- Publication Type:Journal Article
- MeSH: CDC2 Protein Kinase; analysis; Cyclin B; analysis; Humans; Immunoblotting; Maturation-Promoting Factor; analysis; Mouth; chemistry; Mouth Neoplasms; chemistry; Protein Kinase C; physiology
- From: Chinese Journal of Stomatology 2002;37(2):123-125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the content and activity of M-phase promoting factor (MPF) in pleomorphic adenoma, mucoepidermoid carcinoma, buccal carcinoma and normal tissue, in order to evaluate the role of MPF in the development of tumor and the relationship between MPF and malignant degree.
METHODSThe content and activity of MPF were assessed by immunobloting and Gollicano method.
RESULTSThe cdc2 and cyclinB (two subunits of MPF) were found both in normal and tumor tissues, and their content in tumor was higher than normal tissues. Buccal carcinoma was 64% higher than normal tissues. The activity of MPF in carcinoma was higher than normal tissue and had positive relation with the malignant extent.
CONCLUSIONSThe content and activity of MPF in tumor are higher than normal tissue. PKC can activate MPF. These results show PKC may promote tumor proliferation by activating MPF and also, the activity of MPF has some relation with malignant extent.
