Intranasal immunization against dental caries with plasmid DNA encoding pac gene of Streptococcus mutans in gnotobiotic rats.
- Author:
Jihua GUO
1
;
Mingwen FAN
;
Zhuan BIAN
;
Rong JIA
;
Bin PENG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Intranasal; Animals; Antibodies, Bacterial; blood; Bacterial Proteins; genetics; Dental Caries; prevention & control; Female; Immunization; Immunoglobulin A; blood; Immunoglobulin G; blood; Membrane Glycoproteins; Plasmids; genetics; Rats; Rats, Sprague-Dawley; Streptococcal Vaccines; genetics; immunology; therapeutic use; Streptococcus mutans; genetics; immunology; Treatment Outcome; Vaccines, DNA; genetics; immunology; therapeutic use
- From: Chinese Journal of Stomatology 2002;37(6):452-455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo assess the efficacy of plasmid DNA encoding pac gene of Streptococcus mutans (S. mutans) intranasally immunized in gnotobiotic rats and to compare the effect of two different delivery systems.
METHODSSprague Dawley rats, infected with S. mutans at 20 days of age, were intranasally immunized with plasmid pCIA-P (group A), Dosper-DNA complex (group B), Bupivacaine-DNA complex (group C). Control rats were either immunized with plasmid pCI (group D), distilled water (group E) or immunized intramuscularly (group F). All the rats were boosted 2 weeks later. ELISA determined the antibodies against the vaccines. Keyes caries score was used to evaluate the anti- caries effectiveness of the vaccines at the terminal study.
RESULTSAs for the antibody reactions, there were significantly (P < 0.01) differences between rats immunized with DNA vaccine and non-immunized rats. And rats in group B and C had the significantly (P < 0.01) higher level of specific salivary anti-PAc IgA antibodies and rats (group B, C, F) had the significantly (P < 0.01) higher specific serum anti-PAc IgG responses to DNA vaccine. Keyes scores of rats (group B and C) were significantly (P < 0.01) lower than others.
CONCLUSIONSIntranasal immunization with plasmid pCIA-P encoding pac gene successfully reduces the caries and appears to be a promising approach against dental caries. Cationic liposome Dosper and local anesthetic bupivacaine could enhance the efficacy of DNA vaccine.
