Transfection of the nm23-H1 gene into BcaCD885 cell line inhibits the potential of invasion, adhesion and mobility.
- Author:
Shao-wei CHEN
1
;
Yu-ming WEN
;
Long-jiang LI
;
Jian PAN
;
Chang-mei WANG
;
Xiang-ling LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; physiology; Cell Movement; physiology; Genetic Vectors; genetics; Humans; Monomeric GTP-Binding Proteins; genetics; metabolism; Mouth Neoplasms; genetics; pathology; physiopathology; NM23 Nucleoside Diphosphate Kinases; Nucleoside-Diphosphate Kinase; Transcription Factors; genetics; metabolism; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Stomatology 2003;38(1):16-19
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo transfect nm23-H1 into the BcaCD885 cell lines in order to get safe high-efficiency and low-toxicity, and to find out whether nm23-H1 could affect the invasion and metastases ability of BcaCD885 cell lines.
METHODSLipofect was used to transfect nm23-H1 into BcaCD885 cell lines; immunohistochemistry was used to detect the difference expression of nm23-H1 between transfected and non-transfected cell lines; then transwell-room and wash way were used to detect the difference of invasion and metastases ability between transfected and non-transfected cell lines.
RESULTSPCMV-NEO-BAM system gave the stability expression of nm23-H1; there was significant different NDPKA expression between transfected and non-transfected BcaCD885 cell lines; the invasion and metastases ability of transfected BcaCD885 cell lines decreased obviously.
CONCLUSIONnm23-H1 can inhibit the metastases of BcaCD885 cell lines significantly.
