Investigation of apoptosis mechanism of arsenic trioxide on oral squamous cell carcinoma.
- Author:
Jun GUO
1
;
Zhi-yuan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Arsenicals; pharmacology; Blotting, Western; Carcinoma, Squamous Cell; drug therapy; metabolism; pathology; Caspase 3; Caspases; metabolism; Cell Cycle; drug effects; DNA, Neoplasm; drug effects; metabolism; Flow Cytometry; Humans; Intracellular Membranes; drug effects; physiology; Membrane Potentials; drug effects; Mitochondria; drug effects; physiology; Mouth Neoplasms; drug therapy; metabolism; pathology; Oxides; pharmacology; Poly(ADP-ribose) Polymerases; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; Tumor Cells, Cultured; drug effects; ultrastructure; Tumor Suppressor Protein p53; metabolism
- From: Chinese Journal of Stomatology 2003;38(1):20-23
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo probe the possible mechanism of growth-inhibitory and apoptosis of oral squamous cell carcinoma by arsenic trioxide.
METHODSThe induction of apoptosis in two tongue squamous carcinoma cells treated by arsenic trioxide was investigated. The morphology changes of the cells was observed under electron microscope. The mitochondrial transmembrane potential was detected using rhodamine 123 and flow cytometry. The cell cycle was detected by flow cytometry, and p16, p53, BCL-2, Caspase-3, and PARP changes were examined by western blot.
RESULTS1. The antiproliferative effect on the oral squamous carcinoma cells by arsenic trioxide was carried out through two ways: induction of apoptosis and toxicity damage. 2. Activation of the caspase-3 and PARP, while no changes of p16, p53, BCL-2 occurred. 3. Mitochondrial transmembrane potential collapse and G(2)-M stagnation were correlated with apoptosis of oral squamous cell carcinoma.
CONCLUSIONS1. Tubulins and mitochondria may be the chief action position of arsenic trioxide, which is the start-up factors of mechanism. 2. Activation of the caspase-3 proteolytic pathway may be one of the pivotal ways of apoptosis procedure induced by arsenic trioxide.
