The effect of dentin phosphoprotein on inducing mineralization.

Author: Song-jiang LUO ¹ ; Yu-jing LI ; Ling WAN ; Ying SU
Author Information

1. Beijing Institute of Dental Research, Beijing Hospital for Stomatology, Capital University of Medical Sciences, Beijing 100050, China.
Publication Type:Journal Article
MeSH: Calcium Phosphates; chemistry; metabolism; Dentin; chemistry; metabolism; Dentinogenesis; Humans; Microscopy, Electron, Scanning; Minerals; chemistry; metabolism; Phosphoproteins; chemistry; metabolism; Tooth Calcification; X-Ray Diffraction
From: Chinese Journal of Stomatology 2003;38(1):56-58
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effect of dentin phosphoprotein (DPP) in inducing dentinal mineralization.
METHODSHuman DPP was combined with EAH-Sepharose 4B beads and its function of inducing mineralization was studied in mineralization system in-vitro. The mineral formed on the surface of the beads was analyzed by scanning electron microscopy (SEM) and the structure was analyzed by X-ray diffraction and plasma emission spectrum.
RESULTSThere was mineral formed on the beads with combined DPP and the mineral was calcium phosphates whose ratio of calcium to phosphate was 1.33. The diffractogram of the formed mineral was more similar to hydroxyapatite than to other calcium phosphates.
CONCLUSIONWhen tightly combined with certain support substance, human DPP can induce mineralization.