Influence of super high molecular weight poly D,L-lactic acid on viability and new bone formation of osteoblasts.
- Author:
Shi-cheng WEI
1
;
Qian ZHENG
;
Lei LIU
;
Sheng-wei LI
;
Han-zhang WANG
;
Cheng-dong XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Bone Plates; Bone Screws; Cell Survival; drug effects; Cells, Cultured; Dogs; Lactic Acid; chemistry; pharmacology; Mandibular Fractures; surgery; Microscopy, Electron, Scanning; Molecular Weight; Oral Surgical Procedures; instrumentation; methods; Osteoblasts; cytology; drug effects; ultrastructure; Osteogenesis; drug effects; Polyesters; Polymers; chemistry; pharmacology; Rats
- From: Chinese Journal of Stomatology 2003;38(1):67-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of the viability and new bone formation of osteoblasts by the super high molecular weight poly D,L-lactic acid (SHMW-PDLLA).
METHODS1. The osteoblasts derived from neonatal rat were grown and maintained at steep of SHMW-PDLLA and normal culture medium. The viability and function of the osteoblasts were measured with MTT array. 2. The plate and screws made of SHMW-PDLLA were implanted and fixed at the artificial fractured mandible of dogs. Specimens were gained at 3 and 6 months and examined with macroscopy and SEM.
RESULTS1. There is no significant difference of OD values between the experimental group and the control group (P > 0.05). The SHMW-PDLLA isn't toxic to osteoblast at 1 week and 2 weeks, and the toxicity is 3% at 3 days. 2. There were a lot of new bone formed between the implanted SHMW-PDLLA plate and bone tissues under SEM.
CONCLUSIONSHMW-PDLLA hasn't pathological influence on the viability and new bone formation of osteoblasts and it is feasible in tissue engineering of bone.
