Effects of lead exposure on acid-sensing ion channel in hippocampus of baby-rats.
- Author:
Jin-Ru DI
1
;
Feng-Shan WANG
;
Jing-Shu ZHANG
;
Zhong-Hui LIU
;
Bo-Dong SHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acid Sensing Ion Channels; Animals; Female; Hippocampus; drug effects; metabolism; Lead; toxicity; Nerve Tissue Proteins; genetics; metabolism; Pregnancy; Prenatal Exposure Delayed Effects; genetics; RNA, Messenger; genetics; Rats; Rats, Wistar; Sodium Channels; genetics; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(12):729-732
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of chronic lead exposure on mRNA and protein expression of ASIC1a, ASIC2a, ASIC2b in hippocampus of baby-rats.
METHODSThe Wistar pregnant rats were randomly divided into 3 groups fed with distilled water or lead contained water (0.2% and 1.0% lead acetate) respectively, 5 rats in each group. The lead-exposure ranged from the 0 day of pregnancy to the offspring weaned. Then the baby-rats were fed with lead water like their mothers and killed at postnatal day 8 or 50. Atomic absorption spectrometry was used to determine lead content in the brain. RT-PCR and Western blotting were used to observe mRNA and protein expression of ASIC1a, ASIC2a and ASIC2b in their hippocampus respectively.
RESULTSThe brain lead content of test groups was higher than that of the control group (P < 0.01), and the lead content of the postnatal day 50 was higher than that in postnatal day 8 (P < 0.01). Compared with the control group, ASIC1a mRNA expression of 1.0% lead exposure in the hippocampus was uptrend (P < 0.01), ASIC1a protein expression of each test group was downtrend (P < 0.05), while for ASIC2a and ASIC2b mRNA and protein, there was no significant differences observed (P > 0.05).
CONCLUSIONASIC1a expression in hippocampus can be changed by chronic lead exposure.
