Relaxant effects of Aike Mixture on isolated bladder and prostatic urethral smooth muscle of rabbits.
- Author:
Min-jian ZHANG
1
;
Ya-lei SHI
;
Wan-jun CHENG
;
Zhen-wei LI
;
Xiang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Chloride; pharmacology; Carbachol; pharmacology; Drugs, Chinese Herbal; pharmacology; Hydroxylamines; pharmacology; In Vitro Techniques; Male; Muscle Contraction; drug effects; Muscle, Smooth; drug effects; physiology; Neuromuscular Agents; pharmacology; Prostate; drug effects; physiology; Rabbits; Receptors, Adrenergic, alpha-1; metabolism; Receptors, Muscarinic; metabolism; Urethra; drug effects; physiology; Urinary Bladder; drug effects; physiology
- From: Chinese journal of integrative medicine 2014;20(6):420-424
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo observe the relaxant effect of Aike Mixture (AKM) on isolated bladder and prostatic urethral smooth muscle of rabbits.
METHODSThe isolated bladder and prostatic urethral smooth muscle from male rabbits were placed in a Magnus bath and smooth muscle contraction was measured using a biological signal acquisition and analysis system. The effects of AKM in combination with methoxyamine, carbachol and CaCl2 on the contractile tension of muscle strips were determined by cumulative dosing.
RESULTSAKM dose-dependently reduced contractile tension of bladder trigone smooth muscle (r=0.831, P<0.05), reduced contractile wave amplitude (r=0.837, P<0.05) and decreased contractile frequency (r=-0.917, P<0.01). AKM significantly inhibited the increases in smooth muscle contraction induced by methoxyamine, carbachol and CaCl2.
CONCLUSIONAKM dose-dependently inhibited the contraction of rabbit isolated bladder and prostatic urethral smooth muscle by antagonizing α1-adrenergic receptors and M-cholinergic receptors.
