A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction on endothelial cells.
- Author:
Jun SONG
1
;
Wen-Yuan CHEN
;
Li-Ya WU
;
Liang-Pu ZHENG
;
Wei LIN
;
Dong GAO
;
Ted J KAPTCHUK
;
Ke-Ji CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inducing Agents; pharmacology; Cell Adhesion; drug effects; genetics; Cell Line; Cell Movement; drug effects; genetics; Cell Proliferation; drug effects; Cell Survival; drug effects; genetics; Drugs, Chinese Herbal; pharmacology; Endothelial Cells; drug effects; metabolism; Gene Expression Regulation; Humans; Neovascularization, Physiologic; drug effects; genetics; Oligonucleotide Array Sequence Analysis; methods
- From: Chinese journal of integrative medicine 2012;18(7):502-506
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction () on the endothelial cell line ECV304.
METHODSECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction-containing serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.
RESULTSAt 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed.
CONCLUSIONXFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.
