Determination of chelerythrine in Chelidonium majus by RP-HPLC.
- Author:
Nan SUN
1
;
Liming YU
Author Information
- Publication Type:Journal Article
- MeSH: Benzophenanthridines; analysis; Chelidonium; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis
- From: China Journal of Chinese Materia Medica 2009;34(22):2918-2919
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.
METHODChelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.
RESULTThe linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.
CONCLUSIONThe method is accurate, sensitive and reliable.
