Preliminary study to investigate dynamic extract process of Huanglian Jiedu Tang and the mechanism of subsidence produce.
- Author:
Linmei PAN
1
;
Jia FU
;
Huaxu ZHU
;
Liwei GUO
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; isolation & purification; Berberine; analogs & derivatives; isolation & purification; Berberine Alkaloids; isolation & purification; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Flavones; isolation & purification; Flavonoids; isolation & purification; Fractional Precipitation; Iridoids; isolation & purification; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the phenomenon of subsidence emergence in the process of Huanglian Jiedu Tang decoction extraction, and the mechanism of subsidence emergence.
METHODUV was applied to determine the concentration of total alkaloids and total flavones; Simultaneous determination of berberine, jatrorrhizine, palmatine, baicalin and geniposide were carried out by HPLC; The solid holdup and precipitation rate were calculated by the formula. The relativity among these parameters was analyzed by the SPSS software program.
RESULTThe contains of berberine, jatrorrhizine, palmatine in total alkaloids showed a good correlation with total alkaloids. The correlation between baicalin and total flavones was lower than that between geniposide and total flavones. Compared to precipitation rate, solided hold up shows a larger relevance with index component
CONCLUSIONWith the change of time, the total alkaloids represented by berberine alkaloids and baicalin at a certain concentration can be regarded as the equilibrium point, or one generated by the critical point of precipitation, the reaction can generate "sediments"; because "precipitation objects" generated, reducing the concentration of the above-mentioned components, destroy the" balance". The relevant components of herbal medicine increased dissolution rate, at the same time might partly dissolved sediment, reaching a new equilibrium state.