Pressurized liquid extraction of Tripterygiumw wilfordii polyglycosides and optimization of extraction conditions.

Author: Lei YANG ¹ ; Tong LI ; Yuangang ZU
Author Information

1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Publication Type:Journal Article
MeSH: Glycosides; isolation & purification; Plant Extracts; chemistry; Pressure; Tripterygium; chemistry
From: China Journal of Chinese Materia Medica 2010;35(1):44-48
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the extraction method of polyglycosides from Tripterygium wilfordii.
METHODThe extraction method of pressurized liquid extraction was employed and chromogenic colorimetric technique were used for quantitative analysis. Based on the single-factor experiment, according to the center combination design this paper used three factors and three levels of response surface methodology for process optimization.
RESULTThe optimized conditions were as follows: ratio of solid to liquid was 1:9.5, at the temperature of 115 degrees C for 80 minutes, the actual extract ratio and purity of polyglycosides obtained were 0.21% and 0.52%, respectively.
CONCLUSIONThe method of pressurized liquid extraction has obvious advantages over conventional reflux extracting.