Study on IgG promoter as probe to evaluate safety of injections in pre-clinic.
- Author:
Yugang WANG
1
;
Lele LI
;
Fan LEI
;
Aihua LIANG
;
Dongming XING
;
Lijun DU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Gene Expression; drug effects; Green Fluorescent Proteins; genetics; metabolism; Humans; Immunoglobulin G; genetics; Injections; Male; Plant Extracts; pharmacology; Plants, Medicinal; chemistry; Plasmids; genetics; Promoter Regions, Genetic; genetics
- From: China Journal of Chinese Materia Medica 2010;35(1):76-79
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct an effective system screening and evaluating possible injections and components inducing allergy type II.
METHODTransfect IgG promoter-regulated green fluorescent protein expressing plasmid into RPMI-8226 cell. The number of fluorescent cells after drug treatment was calculated and statistically evaluated.
RESULTTween 80 can suppress the expression of IgG effectively, and puerarin has activity of stimulating IgG expression, and the system has no response to KCl-treatment. No effect of Yuxingcao (Herba Houttuyniae) injection on this system is observed.
CONCLUSIONIgG promoter-drove green fluorescent protein expressing cell line can be used as system screening injections and components inducing allergy type II based on stimulating IgG promoter activity. Tween 80 can suppress the expression of IgG at the transcriptional level. Four batches of Yuxingcao injection cannot induce allergy type II by activating IgG expression.
