Effects of methyl protodioscin on Ca2+i and ATPase activity in cardiomyocytes and analysis of mechanisms.
- Author:
Zong NING
1
;
Yikui LI
;
Rongli ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ca(2+) Mg(2+)-ATPase; metabolism; Calcium; metabolism; Calcium Channels; drug effects; Cell Membrane; drug effects; metabolism; Cells, Cultured; Diltiazem; pharmacology; Diosgenin; analogs & derivatives; pharmacology; Enzyme Activation; drug effects; Female; Male; Myocytes, Cardiac; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Saponins; pharmacology; Sarcoplasmic Reticulum Calcium-Transporting ATPases; metabolism; Sodium-Potassium-Exchanging ATPase; drug effects
- From: China Journal of Chinese Materia Medica 2010;35(1):80-83
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of methyl protodioscin on the [Ca2+]i and the ATPase activity in cardiomyocytes, as well as their mechanisms.
METHODThe cardiomyocytes were randomly divided into three groups, the control group treated with no serumal DMEM, the MPD group treated with MPD and the dilthiazem group treated with dilthiazem. Fluorospectrophotometer was used to determined the level of myocardial cell intracellular Ca2+ [Ca2+]i. In the experiment of ATPase activity on cellular membrane, the cardiomyocytes were randomly divided into two groups, the control group treated with no serumal DMEM, the MPD group treated with MPD. The activity of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and Mg2+-ATP ATPase were determined. The quantitative analysis of SERCA2a mRNA expression was studied by RT-PCR that the groups and treatments in cardiomyocytes same as the experiment for ATPase activity assay.
RESULTUnder the quiescent condition, compared to the control group, the level of [Ca2+]i in cardiomyocytes of the MPD group and dilthiazem group was no different. After treatment with 40 mmol x L(-1) KCl, [Ca2+] was significantly lower in the MPD group and the dilthiazem group, and the intensity of peak value in time course of 60 s, the dilthiazem group and the MPD group also were lower than the control group (P < 0.001). Ca2+-Mg2+-ATPase and Na+-K+-ATPase in cultured rat were increased after treated with MPD compared to treatment with no serumal DMEM (P < 0.05, P < 0.01), but Mg2+-ATPase in these groups had no different. The expression of SERCA2a mRNA between the MPD group and the control group was no different. MPD could not up-regulated or down-regulated SERCA2a in endocytoplasmic reticulum.
CONCLUSIONMethyl protodioscin could block the volt dependent form calcium channel in cellular membrane, and up-regulate the function of sodium pump and calcium pump, so that it could remain low calcium in the internal environment in cardiomyocytes.
