Protective effects of resveratrol on neonatal rat cardiomyocyte lesion induced by hypoxia.
- Author:
Xiulan HUANG
1
;
Qiulan ZHOU
;
Lili GU
;
Danni LIU
;
Zhiyong LI
;
Qingshan LIU
;
Dan ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Hypoxia; physiology; Cells, Cultured; Dose-Response Relationship, Drug; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; L-Lactate Dehydrogenase; metabolism; Myocytes, Cardiac; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Stilbenes; pharmacology
- From: China Journal of Chinese Materia Medica 2010;35(1):94-98
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of resveratrol (Res) on neonatal rat cardiomyocyte lesion induced by hypoxia.
METHODThe cardiomyocyte of neonatal rats were cultured in vitro and the model of cardiomyocyte hypoxia was established. The cardiomyocyte vitalities were determined by MTT assay, the HIF-1alpha expression levels in myocardial cells was detected by immunohistochemical, the activities of peroxidase (GSH-Px) and lactate dehydrogenase (LDH) were measured as well.
RESULTAfter the administration of hypoxia for 24 hours, the HIF-1alpha expression in myocardial cells was significantly increased. The LDH level in the culture medium was increased from (93.07 +/- 15.84) U x L(-1) to (750.77 +/- 181.51) U x L(-1) (P < 0.01). The intracellular GSH-Px activity was decreased from (46.96 +/- 8.36) U x mL(-1) to (27.13 +/- 4.76) U x mL(-1) (P < 0.05). Res 25, 50 and 75 micromol x L(-1) could dose-dependently inhibit the raising of the HIF-1alpha expression in myocardial cells induced by hypoxia. The LDH activities were decreased dose-dependently to (486.17 +/- 69.97), (189.43 +/- 32.07), (155.34 +/- 29.57) U x L(-1), respectively (P <0.05 or P <0.01). The GSH-Px activities were increased dose-dependently (33.55 +/- 6.34), (37.67 +/- 6.73), (41.44 +/- 7.91) U x mL(-1) (P < 0.05 or P < 0.01).
CONCLUSIONRes has a protective effect on neonatal rat cardiomyocyte lesion induced by hypoxia.
