Effects of cryptotanshinone on proliferation and apoptosis of Hela cell line of cervical cancer.
- Author:
Yintao YE
1
;
Wenqing XU
;
Wei ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Blotting, Western; Cell Cycle; drug effects; Cell Proliferation; drug effects; Drugs, Chinese Herbal; therapeutic use; Female; Flow Cytometry; HeLa Cells; Humans; Oncogene Proteins, Viral; metabolism; Phenanthrenes; therapeutic use; Repressor Proteins; metabolism; Tumor Suppressor Protein p53; metabolism; Uterine Cervical Neoplasms; drug therapy
- From: China Journal of Chinese Materia Medica 2010;35(1):118-121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the proliferation effects and apoptosis induction of cryptotanshinone on Hela cell line of cervical cancer.
METHODThe MTT assay was used to detect the growth inhibition rates of Hela cells at 24, 48, 72 h which cultured with cryptotanshinone in different concentrations. The cell cycle distribution and apoptosis were measured by flow cytometry. The protein expressions of E6, p53 and p21 were studied by Western blot.
RESULTThe different concentrations (0.5-16 mg x L(-1)) of cryptotanshinone had cytotoxicity on Hela cells, which were clearly dose and time-dependent. The IC50 of 24, 48, 72 h were 17.8, 8.17, 6.55 mg x L(-1), respectively. Cells were treated with cryptotanshinone which had significant effects on cell cycle of Hela cell, and induced apoptosis. Western blot showed cryptotanshinone decreased expressions of HPV E6 and increased expressions of p53 and p21 proteins.
CONCLUSIONCryptotanshinone had significant cytotoic and radiosensitization effects on cervical cancer Hela cells. One of the mechanism may be that it might make significant G0/G1 phase arrest and induced apoptosis, a decrease in S phase, and restore the function of p53 to induce apoptosis in Hela cells to kill the tumor cell.
