ISSR analysis of genetic diversity of Coptis deltoidea.

Chunping Zhang; Ping HE; Shijun HU; Fenggang Yuan; Ruibo Wang; Shan Gao

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ISSR analysis of genetic diversity of Coptis deltoidea.

Author: Chunping ZHANG ¹ ; Ping HE ; Shijun HU ; Fenggang YUAN ; Ruibo WANG ; Shan GAO
Author Information

1. School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China.
Publication Type:Journal Article
MeSH: Cluster Analysis; Coptis; classification; genetics; DNA, Plant; genetics; Genetic Variation; genetics; Genetics, Population; Phylogeny; Polymorphism, Genetic; genetics
From: China Journal of Chinese Materia Medica 2009;34(24):3176-3179
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the genetic diversity of Coptis deltoidea.
METHODThe genetic diversity of 90 individuals from 8 populations was analyzed by ISSR.
RESULTTwelve primers were selected to produce highly reproducible ISSR bands. Among 128 amplified bands, 94 showed polymorphism, the percentage of polymorphic bands reached 73.44%. Nei's gene diversity index (H) was 0.1925, Shannon's information index (I) was 0.3028, Gst was 0.7212. The genetic distance coefficient and similarity were 0.0858-0.2314 and 0.8046-0.9425, respectively.
CONCLUSIONC. deltoidea held a high genetic diversity and the majority of genetic variation occurs among populations. By cluster analysis, the geographical distribution is very obvious. The ISSR marker can be used for the analysis of genetic diversity and genetic variation of C. deltoidea.