Identification of corium stomachium galli by molecular taxonomy.
- Author:
Meng QU
1
;
Jichun CUI
;
Zhiheng DONG
;
Lihua ZHANG
;
Bingmei WANG
;
Jilin ZHANG
;
Cai LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chickens; classification; genetics; DNA, Mitochondrial; genetics; Liver; metabolism; Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2009;34(24):3192-3194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a convenient and accurate method of DNA molecular marker for the identification of corium stomachium galli.
METHODCytb mtDNA sequences of Gallus gallus domestica and three other species of poultry were downloaded from Genbank. Species-specific PCR primers were designed according to the differential DNA fragments of the cytb genes. PCR tests were performed with the DNAs extracted from G. gallus domestica, three other poultry species and domestic mammal animals.
RESULTThe specific primers of G. gallus domestica could only amplify the cytb mtDNA of G. gallus domestica.
CONCLUSIONThe primers are specific to G. gallus domestica mtDNA and can used to discern Corium stomachium from the false medicine.