Regulating effects of aquaporin 4 expression in LoVo cells by serum containing total anthraquinone in rheum.
- Author:
Qing LIU
1
;
Feng LI
;
Junchang LI
;
Jufeng YAO
;
Xin WANG
;
Changhai WANG
;
Wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anthraquinones; blood; pharmacology; Aquaporin 4; genetics; metabolism; Blotting, Western; Cell Line, Tumor; Gene Expression; drug effects; Humans; Male; Random Allocation; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Rheum; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(24):3247-3250
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulating effects of serum containing total anthraquinone in rheum on the expression of AQP4 in cultured LoVo cells in vitro.
METHODSixteen SD rats were randomly divided into 4 groups respectively treated with 0, 1.4, 2.5, 4.5 g x kg(-1) x d(-1) of total anthraquinone in rheum by intragastric administration. After 7 days of treatment, the rats were anesthetized and celiotomized to prepare the serum containing total anthraquinone in rheum. LoVo cells cultured in vitro were treated with different concentrations of total anthraquinone in rheum for 24 h. The expression levels of protein and mRNA of AQP4 IN LoVo cells were decided by Western blot and semiquantive RT-PCR.
RESULTWestern blot and semiquantive RT-PCR showed that serum containing total anthraquinone in rheum prepared from rats treated with 4.5 g x kg(-1) x d(-1) of total anthraquinone in rheum could inhibit the expression of mRNA and protein levels of AQP 4 in LoVo cells (P<0.01).
CONCLUSIONSerum containing total anthraquinone in rheum can inhibit both the transcription and translation of AQP4 gene, indicating that the regulation mechanism of rhubarb on the expression of AQP4 can be related to the cathartic effect of rhubarb.
