Comparison of anti-inflammatory effect and analysis of astilbin red and white transverse section Smilax glabra in 28 collection sites.
- Author:
Xi-Cheng HE
1
;
Qin-Wen SHUN
;
Li-Sha DONG
;
Xiang-Qian GE
;
Hua ZHANG
;
Heng NONG
;
Xiao-Hua SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; chemistry; pharmacology; China; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; pharmacology; Female; Flavonols; chemistry; pharmacology; Male; Mice; Permeability; Smilax; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(23):3595-3598
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEAstilbin in 28 Smilax glabra (red and white cross-section) from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment (penetration type).
METHODThe analysis was performed on a Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0. 1% acetic acid aqueous (21: 79) at a flow rate of 1.0 mL x min(-1). The detection wavelength was 291 nm, and the column temperature was 25 degrees C. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment.
RESULTLinear correlation was good in the range of 0.003 379-4.004 microg, and the average recoveries were 100.1%, 101.9%, 99.3%, respectively. The content of astilbin in white and red transverse section were 0.19%-2.46% and 2.10%-5.92%, respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%, respectively.
CONCLUSIONAstilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra, the content of astilbin is not positively related with anti-inflammatory effect.
