OBJECTIVETo observe the effect of Jinhuang Fuzheng powder on cytokines of immunosuppressive mice by using protein antibody micro-array.

METHODThe immunosuppressive mice model was established by subcutaneously injecting cyclophosphamide. Rats were orally administered with low, middle and high dose of Huangjin Fuzheng powder for 10 days, and fasted for 12 hours after the final administration. 1 mL blood was drawn from caudal veins and isolated hearts of rats of each group. A quantitative test was conducted for cytokines with cytokine antibody array.

RESULTCompared with the control group, IFN-gamma and RANTES of the CTX group decreased significantly (P<0.05). After the administration, IFN-gamma of low, middle and high-dose groups, and RANTES of the high-dose group increased significantly (P<0.05). Compared with the control group, IL-5, IL-6, IL-9, IL-13 and MCP-1 of the CTX group increased remarkably (P<0.01, P<0.05). After the administration, IL-5, IL-6, IL-9, IL-13 and MCP-1 of low, middle and high-dose groups decreased to varying degrees. GM-CSF, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-10, IL-12, IL-17, M-CSF, TNF-alpha, KC and VEGF of the 13 types of cytokines showed no significant change.

CONCLUSIONJinhuang Fuzheng powder shows effect on 20 types of cytokines of immunosuppressive mice to varying degrees, which may be related to the regulatory immunosuppression of Th1/Th2 subgroup in mice.