Improvement of carbon tetrachloride drug-induced liver injury model in vitro.
- Author:
Hai-Jian WU
1
;
Xiu GONG
;
Yi-Tian YANG
;
Yu-Zhuo WANG
;
Xia LI
;
Hua XIN
;
Li-Kuan HU
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; metabolism; Aspartate Aminotransferases; metabolism; Carbon Tetrachloride; adverse effects; Chemical and Drug Induced Liver Injury; enzymology; etiology; Hep G2 Cells; Humans; Liver; drug effects; enzymology; injuries; Models, Biological; Superoxide Dismutase; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(23):3633-3636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an ideal CCl4 drug-induced liver injury model in vitro.
METHODTraditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase (ALT) in supernatant was detected by biochemical method. 4-Methyl-tetrazolium (MTT) chromatometry was adopted for determining cell activity.
RESULTThe improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect.
CONCLUSIONThe improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies.
