Pharmacokinetic study on three main ingredients of refined coronary cataplasm.
- Author:
Rui HOU
1
;
Lan WANG
;
Mao-Bo DU
;
Ri-Xin LIANG
;
Shu-Zhi LIU
;
Yan-Li WANG
;
Jian-Yong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzoates; administration & dosage; pharmacokinetics; Benzofurans; administration & dosage; pharmacokinetics; Bridged-Ring Compounds; administration & dosage; pharmacokinetics; Chromatography, Liquid; methods; Coronary Disease; drug therapy; Diterpenes, Abietane; administration & dosage; pharmacokinetics; Drugs, Chinese Herbal; administration & dosage; pharmacokinetics; Glucosides; administration & dosage; pharmacokinetics; Humans; Male; Monoterpenes; Rabbits; Tandem Mass Spectrometry; methods
- From: China Journal of Chinese Materia Medica 2012;37(23):3641-3646
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a LC-MS/MS method for determining the concentration of tanshinone IIA, salvianolic acid B and paeoniflorin of refined coronary cataplasm in rabbit plasma, in order to determine the concentration of the three main ingredients in blood after transdermal administration and calculate their pharmacokinetic parameters.
METHODRabbits were given refined coronary cataplasm on the basis of 15 g x kg(-1) by transdermal administration to detect the plasma concentration of the three main ingredients using LC-MS/MS. Winnonlin software was used to calculate their major pharmacokinetic parameters.
RESULTTanshinone IIA, salvianolic acid B and paeoniflorin showed good linearity (r>0.999) at 1-100, 50-1 000, 10-1 000 microg x L(-1) respectively in plasma, with average recovery rate of 96.57%, 91.90%, 95.93%, respectively. The RSD within day were less than 15%. After transdermal administration of refined coronary cataplasm in rabbits, the main pharmacokinetic parameters of tanshinone IIA, salvianolic acid B or paeoniflorin were as follows: Cmax (20.85 +/- 12.68), (636.25 +/- 386.91), (787.80 +/- 395.64) microg x L(-1); Tmax (0.49 +/- 0.28), (0.44 +/- 0.27), (0.46 +/- 0.30) h.
CONCLUSIONThe LC-MS/MS method is highly selective and sensitive to determine the concentration of samples in rabbit plasma. The pharmacokinetic characteristics of tanshinone IIA, salvianolic acid B and paeoniflorin are suitable to assess the percutaneous absorption of refined coronary cataplasm.
