HPLC determination of hydroxysafflor yellow A in Mongolian medicine Dedu Honghuaqiwei pill.
- Author:
Chang-Lian CHEN
1
;
Si-Le HU
Author Information
- Publication Type:Journal Article
- MeSH: Chalcone; analogs & derivatives; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Medicine, Mongolian Traditional; Quinones; analysis
- From: China Journal of Chinese Materia Medica 2012;37(23):3673-3675
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the determination of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.
METHODThe determination was performed by HPLC method on Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column at 403 nm using methanol-acetonitrile-0.7% phosphoric acid-water (26: 2: 72) as mobile phase. The column temperature was 30 degrees C and the flow rate was 1.0 mL x min(-1).
RESULTThe linear rang of hydroxysafflor yellow A was 0.068-0.408 microg and the recovery was 97.66%.
CONCLUSIONThe result is accurate with good resolution, and the established method can be applied to determine the content of hydroxysafflor yellow A in Dedu Honghuaqiwei pill.
