Determination of dehydroabietic acid and abietic acid in aqueous alkali extract of Liquidambaris Resina by HPLC.
- Author:
Ying-Feng WANG
1
;
Xiao-Yan WEI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Diterpenes, Abietane; analysis; Drugs, Chinese Herbal; analysis; Liquidambar; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(1):57-59
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for content determination of dehydroabietic acid and abietic acid in aqueous alkali extract of Liquidambaris Resina.
METHODThe determination was carried out on a DIONEX C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% acetic acid. The flow rate was 1 mL x min(-1), and the detected wavelength was set at 210, 240 nm.
RESULTThe peak areas and the sample quantity of the two components had good linear relationship in the range of 0.4-3.4 microg for dehydroabietic acid, and 0.6-4.8 microg for abietic acid. The average recoveries were 99.53%, 101.9%, respectively.
CONCLUSIONThe method was proved to be simple, accurate and used for the quality evaluation of Liquidambaris Resina.
