Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification.
- Author:
Li-Li WANG
1
;
Hui-Fang LV
;
Li ZHANG
;
Hai-Xia HUA
;
Jie-Hua WANG
;
Zhi-Bi HU
;
Wan-Kui LI
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcholinesterase; metabolism; Acremonium; genetics; metabolism; Cholinesterase Inhibitors; isolation & purification; metabolism; Chromatography, Thin Layer; DNA, Fungal; chemistry; genetics; DNA, Ribosomal; chemistry; genetics; Diazonium Compounds; metabolism; Fungi; classification; genetics; metabolism; Huperzia; microbiology; Hydrolysis; Naphthaleneacetic Acids; metabolism; Phylogeny; RNA, Ribosomal, 18S; classification; genetics; RNA, Ribosomal, 5.8S; classification; genetics; Sequence Analysis, DNA
- From: China Journal of Chinese Materia Medica 2012;37(24):3701-3705
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen out fungus strains with acetylcholinesterase inhibitory activity from Huperzia serrata.
METHODEndophytic fungi fermentation products from 59 H. serrata strains were stained with acetylcholinesterase hydrolyzed alpha-naphthaleneacetic ethyl ester and fast blue B salt, and screened for acetylcholinesterase inhibitory activity with thin-layer chromatography-bioautography. Target strains were classified and identified through the sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics.
RESULTFungus strain LQ2F01 from H. serrata showed positive color reaction in the screening for acetylcholinesterase inhibitory activity. The sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics showed the strain LQ2F01 belonged to Acremonium.
CONCLUSIONEndophytic Fungi LQ2F01 from H. serrata shows identical acetylcholinesterase inhibitory activity with the host plant, which is of great significance to the development of natural medicines and the studies on the relationship between the endophytic gungi and the host plant.
