Cultivation of Panax ginseng adventitious roots in bubble bioreactors.
- Author:
Bei-Mei ZUO
1
;
Wen-Yuan GAO
;
Juan WANG
;
Shuang-Shuang YIN
;
Hui LIU
;
Li-Ming ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Biomass; Bioreactors; classification; standards; Chromatography, High Pressure Liquid; Ginsenosides; metabolism; Panax; genetics; metabolism; Plant Roots; genetics; metabolism; Polysaccharides; metabolism; Reproducibility of Results; Spectrophotometry, Ultraviolet; Time Factors; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2012;37(24):3706-3711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study cultivation of Panax ginseng adventitious roots in bubble bioreactors.
METHODThe adventitious roots were obtained through tissue culture different types of bioreactors. The contents of ginsenosides Re, Rb1 and Rg1 were determined by HPLC while the contents of polysaccharides were determined by ultraviolet spectrophotometry.
RESULTThe results showed that of the three types tested, the most efficient bioreactor for cultivation of the ginseng adventitious roots was the cone-type bioreactors (with the 120 degrees ), in which, the growth curve of adventitious roots was S-shaped. The maximum biomass was obtained on the 40th day, with the fresh weight, dry weight and growth rate reaching the maximum, which were 113.15 g, 9.62 g and 63.13 times respectively, and the concomitant contents of polysaccharide and ginsenoside were 2.73% and 2.25 mg x g(-1).
CONCLUSIONThe results showed that the most efficient bioreactor for cultivation of the ginseng adventitious roots was the cone-type bioreactors (with the 120 degrees). These results provide a theoretical reference for developing an efficient production process of active metabolites of ginseng in the scale-up cultivation.
