Induction of adventitious roots of Echinacea pallida and accumulation of caffeic acid derivatives.
- Author:
Chun-Hua WU
1
;
Tao HUANG
;
Xi-Hua CUI
;
Keeyoeup PAEK
Author Information
- Publication Type:Journal Article
- MeSH: 2,4-Dichlorophenoxyacetic Acid; pharmacology; Bioreactors; Caffeic Acids; chemistry; metabolism; Dose-Response Relationship, Drug; Echinacea; drug effects; growth & development; metabolism; Indoleacetic Acids; pharmacology; Indoles; pharmacology; Naphthaleneacetic Acids; pharmacology; Plant Growth Regulators; pharmacology; Plant Leaves; drug effects; growth & development; metabolism; Plant Roots; drug effects; growth & development; metabolism; Seedlings; drug effects; growth & development; metabolism; Tissue Culture Techniques; instrumentation; methods
- From: China Journal of Chinese Materia Medica 2012;37(24):3768-3772
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of auxins 2,4-D,IAA,IBA,NAA on induction of adventitious roots as well as that of IBA concentrations on the growth of adventitious roots and the accumulation of caffeic acid derivatives, with test-tube seedling leaves Echinacea pallida as the explant, and cultivate adventitious roots in bioreactors.
RESULT1.0 mg x L(-1) IBA was found the best for the induction of adventitious roots, with the numer of induced adventitious roots up to 22. 5 in each culture dish. Among different concentrations for suspension cultivation of IBA tested, 1.0 mg x L(-1) IBA was found the most suitable for the growth of adventitious roots and the accumulation of caffeic acid derivatives. In a 5 L balloon type bubble bioreactor, 8.98 g x L(-1) dry weight was achieved after one month, which was 2.05 times of 4.38 g x L(-1) dry weight cultivated in a triangular flask. The content of echinacoside cultivated in a bioreactor was 14.08 mg x g(-1) DW, which was 2.4 times of cultivated roots. The contents of chlorogenic acid, chicoric acid and total caffeic acid derivatives were 4.0-25.6 times of ultivated roots.
CONCLUSIONThe study can provide high-quality biomedical drugs containing such caffeic acid derivatives as echinacoside for mass production of Echinacea purpurea medicines.
