Molecular identification of astragali radix and its adulterants by ITS sequences.
- Author:
Zhan-Hu CUI
1
;
Yue LI
;
Qing-Jun YUAN
;
Li-She ZHOU
;
Min-Hui LI
Author Information
- Publication Type:Journal Article
- MeSH: Althaea; classification; genetics; Astragalus membranaceus; classification; genetics; DNA, Plant; chemistry; genetics; DNA, Ribosomal; chemistry; genetics; DNA, Ribosomal Spacer; genetics; Fabaceae; classification; genetics; Medicago sativa; classification; genetics; Molecular Sequence Data; Phylogeny; Plant Roots; genetics; RNA, Ribosomal; genetics; RNA, Ribosomal, 5.8S; genetics; Sequence Analysis, DNA; Species Specificity
- From: China Journal of Chinese Materia Medica 2012;37(24):3773-3776
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a new method for identification Astragali Radix from its adulterants by using ITS sequence.
METHODThirteen samples of the different Astragali Radix materials and 6 samples of the adulterants of the roots of Hedysarum polybotrys, Medicago sativa and Althaea rosea were collected. ITS sequence was amplified by PCR and sequenced unidirectionally. The interspecific K-2-P distances of Astragali Radix and its adulterants were calculated, and NJ tree and UPGMA tree were constructed by MEGA 4.
RESULTITS sequences were obtained from 19 samples respectively, there were Astragali Radix 646-650 bp, H. polybotrys 664 bp, Medicago sativa 659 bp, Althaea rosea 728 bp, which were registered in the GenBank. Phylogeny trees reconstruction using NJ and UPGMA analysis based on ITS nucleotide sequences can effectively distinguish Astragali Radix from adulterants.
CONCLUSIONITS sequence can be used to identify Astragali Radix from its adulterants successfully and is an efficient molecular marker for authentication of Astragali Radix and its adulterants.
