Effect of different developmental stage on plant growth and active compounds in Scutellaria baicalensis.
- Author:
Guo-Qiang HU
1
;
Yuan YUAN
;
Chong WU
;
Chao JIANG
;
Zhou-Yong WANG
;
Shu-Fang LIN
;
Zhi-Gang WU
Author Information
- Publication Type:Journal Article
- MeSH: Acyltransferases; genetics; metabolism; Chromatography, High Pressure Liquid; Coenzyme A Ligases; genetics; metabolism; Flavonoids; analysis; metabolism; Flowers; genetics; growth & development; metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glucuronidase; genetics; metabolism; Glucuronosyltransferase; genetics; metabolism; Phenylalanine Ammonia-Lyase; genetics; metabolism; Plant Proteins; genetics; metabolism; Plant Roots; genetics; growth & development; metabolism; Plant Stems; genetics; growth & development; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Scutellaria baicalensis; genetics; growth & development; metabolism; Seedlings; genetics; growth & development; metabolism; Spectrophotometry, Ultraviolet; Time Factors; Trans-Cinnamate 4-Monooxygenase; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(24):3793-3798
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the developmental phase on the growth and active compounds in Scutellaria baicalensis.
METHODSeeds of wild plants were collected from Laiwu and sowed in Fangshan (Beijing) and Laiwu (Shandong). Samples of aerial and underground parts were collected in five growth periods of sprouts, seedlings, flowering, seed drop and withered periods respectively. The length of taproot, fresh weight of root, diameter of taproot and the length of stem were determined. The content of active compounds and total flavonoids were determined by HPLC and ultraviolet spectrophotometry respectively. The transcripted level of PAL1, PAL2, PAL3, C4H, 4CL, CHS, GUS and UBGAT were analyzed with RT-PCR.
RESULTThe results showed that the aerial part of S. baicalensis grew quickly before flowering stage, and the underground part grew mostly between the periods of flowering and withered. In the whole growing developmental periods, the content of total flavonoids was not changed significantly, the content of baicalin was increased gradually and the content of baicalein was decreased gradually. Expression level of PAL and 4CL was the highest in withered period, CHS was increased between flowering and seed drop and decreased in withered period.
CONCLUSIONSeedlings and withered periods may be the key phase affecting the growth and active compounds in S. baicalensis.
