Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus.
- Author:
Gui-Xiang WAN
1
;
Lin MA
;
Jian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: 2,4-Dichlorophenoxyacetic Acid; pharmacology; Benzyl Compounds; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; Flavonoids; metabolism; Kinetin; pharmacology; Naphthaleneacetic Acids; pharmacology; Plant Growth Regulators; pharmacology; Purines; Scutellaria baicalensis; drug effects; metabolism; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2012;37(24):3799-3802
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.
METHODHPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions: ODS column was adopted, with methanol-water-phosphate (47: 53: 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.
RESULTS. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).
CONCLUSIONThe experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.
