Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa.
- Author:
Jian-Ping XUE
1
;
Tao XUE
;
Lan GUO
;
Yan-Fang ZHU
;
He-Dong LU
;
Ai-Min ZHANG
;
Wei SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Benzyl Compounds; Dose-Response Relationship, Drug; Kinetin; pharmacology; Naphthaleneacetic Acids; pharmacology; Plant Growth Regulators; pharmacology; Plant Leaves; drug effects; growth & development; Plant Roots; drug effects; growth & development; Purines; Rehmannia; drug effects; growth & development; Seedlings; drug effects; growth & development; Sucrose; pharmacology; Tissue Culture Techniques; instrumentation; methods
- From: China Journal of Chinese Materia Medica 2012;37(24):3812-3814
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa, in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R. glutinosa.
METHODLeaves from test-tube seedlings of 85-5 R. glutinosa were used as explants. After rooting induction, they were transferred to medium with orthogonal design for inducing test-tube tuberous roots of R. glutinosa.
RESULT AND CONCLUSIONNAA played a significant role in induction of test-tube tuberous roots of R. glutinosa, followed by sucrose and 6-BA. With leaves from test-tube seedlings as the explants, the optimal medium for inducing test-tube tuberous roots of R. glutinosa was MS + BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 7%. The study provides an efficient induction system for studies on artificial seeds and secondary metabolism with test-tube tuberous roots of R. glutinosa.
