Chemical constituents and their antitumor cytotoxic activity in leaves of Ilex centrochinensis.
- Author:
Lu-Jun LI
1
;
Peng DU
;
Ke-Huan SUN
;
Mei-Qun CAO
;
Zheng-Zhi WU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; chemistry; pharmacology; Caco-2 Cells; Cell Line, Tumor; Cell Survival; drug effects; Dose-Response Relationship, Drug; Humans; Ilex; chemistry; Inhibitory Concentration 50; Plant Extracts; chemistry; pharmacology; Plant Leaves; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(3):354-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the chemical constituents in leaves of Ilex centrochinensis and their antitumor bioactivity.
METHODVarious chromatography techniques such as column chromatography on silica gel, Sephadex LH-20 and preparative HPLC were used to isolate and purify the compounds and their structures were identified by spectral data and physicochemical properties. Their antitumor effect was tested by MTT method.
RESULTTen compounds were isolated and identified as 1,4-benzenediol (1), (2S)-5,4'-dihydroxy-7,3'-dimethoxyflavan(2), (2S)-5,4'-dihydroxy-7-methoxyflavan (3), kaempferol (4), quercetin (5), naringenin (6), ursolic acid (7), uvaol (8), oleanolic acid (9) and beta-sitosterols (10).
CONCLUSIONCompounds 1-5, 7, 8 were isolated from the species for the first time, among which compounds 1-3 were isolated from the Ilex genus for the first time. Compounds 2 and 3 showed strong cytotoxic activity against Huh7 cell lines with IC50 values of 8.98, 13.04 mg x L(-1), respectively. Compounds 7-9 exhibited weak cytotoxic activity against Caco-2 cell lines with IC50 values of 28.52, 38.28, 33.04 mg x L(-1), respectively.
