Effect of cordyceps polysaccharide on lipid peroxidation of rats with dimethylnitrosamine-induced liver fibrosis.
- Author:
Jing-Hua PENG
1
;
Xue-Mei LI
;
Yi-Yang HU
;
Qin FENG
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Blotting, Western; Collagen; metabolism; Cordyceps; chemistry; Dimethylnitrosamine; toxicity; Drug Administration Schedule; Glutathione; metabolism; Glutathione Peroxidase; Hydroxyproline; Immunohistochemistry; Lipid Peroxidation; drug effects; Liver; drug effects; metabolism; pathology; Liver Cirrhosis, Experimental; blood; etiology; prevention & control; Male; Malondialdehyde; metabolism; Phytotherapy; Polysaccharides; administration & dosage; pharmacology; Proliferating Cell Nuclear Antigen; metabolism; Rats; Rats, Wistar; Superoxide Dismutase; metabolism; gamma-Glutamyltransferase; blood
- From: China Journal of Chinese Materia Medica 2013;38(3):391-396
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the pharmacological effect of Cordyceps polysaccharide on dimethylnitrosamine (DMN)-induced liver fibrosis in rats.
METHODDMN rat liver fibrosis model was established and divided into the normal group (N, n = 6), the model group (M, n = 11), the Cordyceps polysaccharide group (C, n = 8) and the colchicine group (Q, n = 9). During the modeling for four weeks, Cordyceps polysaccharide (60 mg x kg(-1)) and colchicine (0.1 mg x kg(-1)) were orally administered for three weeks, while the model and normal groups were given disinfected water of the same amount.
OBSERVATIONserum ALT, AST, GGT and Alb, TBil content; content of hydroxyproline (Hyp) in liver tissues; liver pathology and collagen staining; SOD activity and MDA, GSH, GSH-Px in liver tissues; protein expression of proliferating cell nuclear antigen (PCNA) in liver tissues.
RESULTSerum ALT, AST, GGT, TBil significantly increased, and A1b decreased significantly in the model group. Hepatic Hyp significantly increased in the model group, whereas the index remarkably decreased in the Cordyceps polysaccharide group and the colchicine group. HE staining: the structure of normal hepatic lobules was damaged, with hepatocytes tumefaction and proliferation of connective tissues in portal tracts in the model group, while the Cordyceps polysaccharide group and the colchicine group recorded notable reduction in above pathological changes. Collagen staining: the model group showed hepatic lobule fibrous septum and many intact pseudolobules; while the Cordyceps polysaccharide group and the colchicine group witnessed decrease in collagen deposition. The model group showed significant decrease in SOD, GSH-Px and GSH and increase in MDA, whereas the Cordyceps polysaccharide group and the colchicine group recorded notable growth in GSH and GSH-Px. The model group showed significant decrease in protein expression of PCNA in liver tissues, while the Cordyceps polysaccharide group and the colchicine group showed significant reduction.
CONCLUSIONCordyceps polysaccharide can significantly inhibit DMN-induced liver fibrosis and lipid peroxidation in rats.
