Synthesis and identification of rutin complete antigen and analysis its immunogenicity.
- Author:
Xiao-Yan BAO
1
;
Fu-Yuan HE
;
Jiao-Li ZENG
;
Zan-Shao XIA
;
Jun-Lin DENG
;
Qing-Hui SUN
;
Hong LEI
;
Yu-Ran DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibody Specificity; immunology; Antigens; immunology; Cattle; Cross Reactions; immunology; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Immune Sera; immunology; Immunization; Male; Periodic Acid; chemistry; Rabbits; Rutin; chemical synthesis; immunology; Serum Albumin, Bovine; immunology; Solutions; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(3):397-401
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESynthesis and identification of complete antigen of rutin, the traditional Chinese medicine active ingredient, and develop rapid detection of rutin using enzyme-linked immunoassay method (ELISA). Immunogenicity of the complete antigen was also studied.
METHODPrepare the complete antigen by sodium periodate solution and identified by UV scanning and SDS-PAGE test. Male New Zealand white rabbits were immunized by the antigen to obtain the antiserum.
RESULTThe results of UV analysis showed that the coupling ratio of complete antigen is 13: 1. SDS-PAGE display of the artificial antigen was delayed compared with bovine serum protein. The titer of rutin antibody is 1:4 000. The sensitivity of IC50 was 5.37 mg x L(-1), the lowest detection limit was 1 mg x L(-1), the average recovery was 102%, the intra and interspecific RSD were less than 10%, cross-reactivity rate of antibodies and other analogs were less than 1%.
CONCLUSIONRutin complete antigen was synthesized successfully, and the rapid detection of rutin by ELISA method was successfully established.
