Protective effect of medicated serum prepared with taohong siwu tang on hydrogen peroxide-induced human umbilical vein endothelial cells.
- Author:
Zhu-Qing LIU
1
;
Deng-Ke YIN
;
Lan HAN
;
Bai-Kun LI
;
Dai-Yin PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Blotting, Western; Caspase 3; metabolism; Cell Survival; drug effects; Cells, Cultured; Drugs, Chinese Herbal; chemistry; pharmacology; Female; Human Umbilical Vein Endothelial Cells; cytology; drug effects; metabolism; Humans; Hydrogen Peroxide; pharmacology; L-Lactate Dehydrogenase; metabolism; Malondialdehyde; metabolism; Rats; Rats, Sprague-Dawley; Serum; chemistry; Superoxide Dismutase
- From: China Journal of Chinese Materia Medica 2013;38(3):402-406
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of medicated serum prepared with Taohong Siwu Tang on hydrogen peroxide-injured human umbilical vein endothelial cells (HUVECs).
METHODSprague Dawley rats were orally administered with 10 mL x kg(-1) extracts from Taohong Siwu Tang (1.75 g crude drug), twice a day for three days, in order to prepare medicated serum of Taohong Siwu Tang. The effect of medicated serum pre-treated with Taohong Siwu Tang (with concentrations of 5%, 10%, 15%) on reduction of H2O2-induced cell activity was detected MTT. Cell morphological changes were observed under microscope. The effect of medicated serum prepared with Taohong Siwu Tang on the apoptosis and antioxidant capacity of HUVECs was detected with the content of malondialdehyde (MDA) and dehydrogenase (LDH), the activity of superoxide dismutase (SOD) and AO/EB staining. The expression of Caspase-3 was determined by western blot.
RESULTCompared with the blank serum control group, cell were significantly less active after being damaged by H2O2 (400 micromol x L(-1)). Medicated serum could significantly improve the SOD activity, reduce the levels of LDH and MDA, and inhibite the expression of Caspase-3. AO/EB staining and inverted microscope also showed that medicated serum prepared with Taohong Siwu Tang could reduce cell apoptosis induced by H2O2.
CONCLUSIONMedicated serum prepared with Taohong Siwu Tang has significant protective effect on HUVECs injured by H2O2.
