Matrix metalloproteinases regulations of human gingival fibroblasts by Porphyromonas gingivalis with different fimA genotypes
10.3321/j.issn:1002-0098.2008.12.007
- VernacularTitle:不同fimA基因型牙龈卟啉单胞菌对牙龈成纤维细胞基质金属蛋白酶表达的影响
- Author:
Lei ZHAO
1
;
He YANG
;
Ya-Fei WU
;
Yu-Ling OUYANG
;
Shu MENG
Author Information
1. 四川大学华西口腔医学院
- Keywords:
Porphyromonas gingivalis;
Fibroblasts;
Matrix metalloproteinases;
fimA
- From:
Chinese Journal of Stomatology
2008;43(12):727-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of matrix metalloproteinases(MMP)regulations of human gingival fibroblasts(HGF)by challenge of Porphyromonas gingivcdis(Pg)with different fimA genotypes.Methods Pg ATCC 33277(type Ⅰ),WCSP115(type Ⅱ),WCSP1.5(type Ⅲ),W83(type Ⅳ)were assessed for their inductions of MMP-1 and MMP-2 expression in HGF.MMP mRNA levels of HGF were determined by real-time RT-PCR and MMP protein levels in culture supematant were determined by ELISA at different time intervals(1,3,6 and 12 h)following continous co-culture of bacteria with HGF.Results When co-cultured with Pg,the MMP-1 and MMP-2 mRNA and protein expression of HGF significantly increased compared with the negative control group(P<0.01).The group of type Ⅱ showed greater up-regulated than other fimA genotypes in the mRNA and protein expressions of MMP-1 and MMP-2,MMP-1 mRNA[(28.88±3.12)-(231.01±24.99)]and protein[(1.35±0.17)-(3.08±1.20)]μg/L;MMP-2 mRNA[(20.42±2.21)-(188.34±37.37)]and protein[(2.57±0.76)-(18.08±1.15)]μg/L for different time periods;While the group of type Ⅲ was weaker than other fimA genotypes,the level of MMP-1 mRNA was[(5.11±0.55)-(72.84±8.84)]and protein[(0.68±0.13)-(1.46±0.94)]μg/L,MMP-2 mRNA[(4.55±0.55)-(25.75±3.12)]and protein [(2.28±0.93)-(11.22±2.46)]μg/L(P<0.05).Conclusions Pg could induce HGF to over-express MMP,and fimA genotypes of Pg may be related to this pathogenicity,which might indicate fimA genotype is associated with pathogenesis of Pg.
